Genetic Structure of Fishery-Impacted Species in the Northwest Atlantic
To determine population structure of three fishery-impacted species--harbor porpoise, Atlantic cod and winter flounder--in the Northwest Atlantic, through development of microsatellite DNA markers and through DNA sequencing of the highly variable mitochondrial DNA.
Microsatellite markers will be isolated for harbor porpoise and cod through enrichment of genomic DNA libraries. Candidate loci will be screened for variability using a subset of porpoise and cod samples. Fluorescently labeled primers and multiplexing will allow rapid genotyping of all samples on an automated sequencer. DNA sequencing of mt DNA will take place using an ABI373A automated sequencer.
Overexploitation of fish stocks in the northwest Atlantic has led to the near collapse of the groundfish industry. High by-catch rates of the federally protected harbor porpoise may result in it being listed as a threatened species. Accurate knowledge of population structure and exchange rates for harbor porpoise, cod and winter flounder will help the development of effective management plans.
Available from the National Sea Grant Library (use NHU number to search) or NH Sea Grant
- Rosel, P., R. Tiedermann and M. Walton (1999). Genetic evidence for limited trans-Atlantic movements of the harbor porpoise "Phocoena phocoena." Marine Biology 133:583-591.
- Rosel, P., S. Frazier, J. Wang and T. Kocher (1999). Genetic structure of harbour porpoise "Phocoena phocoena" populations in the northwest Atlantic based on mitochondrial and nuclear markers. Molecular Ecology 8:S41-S54.